KLF2 Polyclonal Antibody (CAB16480)

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CAB16480
  • Western blot - KLF2 Polyclonal Antibody (CAB16480)
  • Western blot - KLF2 Polyclonal Antibody (CAB16480)
  • Immunofluorescence - KLF2 Polyclonal Antibody (CAB16480)
  • Immunofluorescence - KLF2 Polyclonal Antibody (CAB16480)
  • Immunofluorescence - KLF2 Polyclonal Antibody (CAB16480)
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Description

Antibody DatasheetAntibody MSDS

KLF2 Polyclonal Antibody (CAB16480)

Overview

Title:KLF2 Polyclonal Antibody (CAB16480)
Size:100µL
Code:CAB16480
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

This gene encodes a protein that belongs to the Kruppel family of transcription factors. The encoded zinc finger protein is expressed early in mammalian development and is found in many different cell types. The protein acts to bind the CACCC box found in the promoter of target genes to activate their transcription. It plays a role in many processes during development and disease including adipogenesis, embryonic erythropoiesis, epithelial integrity, inflammation and t-cell viability.

Immunogen Information

Immunogen:A synthetic peptide corresponding to a sequence within amino acids 50-150 of human KLF2 (NP_057354.1).
GeneID:10365
SwissProt:Q9Y5W3
Synonyms:KLF2; LKLF; Kruppel like factor 2
Calculated MW:37kDa
Observed MW:37kDa

Applications

Reactivity:Human, Mouse, Rat
Tested Applications:WB; IF
Recommended Dilution:WB 1:500 - 1:2000; IF 1:50 - 1:200
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunofluorescence    
Positive Samples:PC-3, rat lung
Cellular Location:Nucleus

Antibody Images

Western blot - KLF2 Polyclonal Antibody (CAB16480)

Western blot analysis of extracts of various cell lines, using KLF2 antibody (CAB16480) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90S.

Western blot - KLF2 Polyclonal Antibody (CAB16480)

Western blot analysis of extracts of various cell lines, using KLF2 antibody (CAB16480) at 1:1000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 3min.

Immunofluorescence - KLF2 Polyclonal Antibody (CAB16480)

Immunofluorescence analysis of C6 cells using KLF2 antibody (CAB16480) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence - KLF2 Polyclonal Antibody (CAB16480)

Immunofluorescence analysis of NIH/3T3 cells using KLF2 antibody (CAB16480) at dilution of 1:100. Blue: DAPI for nuclear staining.

Immunofluorescence - KLF2 Polyclonal Antibody (CAB16480)

Immunofluorescence analysis of U2OS cells using KLF2 antibody (CAB16480) at dilution of 1:100. Blue: DAPI for nuclear staining.

KLF2 Polyclonal Antibody (CAB16480) Protein Information

UniProt Protein Function:KLF2: Binds to the CACCC box in the beta-globin gene promoter and activates transcription. Belongs to the krueppel C2H2-type zinc-finger protein family.
UniProt Protein Details:

Protein type:C2H2-type zinc finger protein; DNA-binding

Chromosomal Location of Human Ortholog: 19p13.11

Molecular Function:protein binding; transcription factor activity

Biological Process: positive regulation of nitric oxide biosynthetic process; positive regulation of protein metabolic process; positive regulation of transcription from RNA polymerase II promoter; positive regulation of transcription, DNA-dependent

NCBI Summary:Kruppel-like factors (KLFs) are a family of broadly expressed zinc finger transcription factors. KLF2 regulates T-cell trafficking by promoting expression of the lipid-binding receptor S1P1 (S1PR1; MIM 601974) and the selectin CD62L (SELL; MIM 153240) (summary by Weinreich et al., 2009 [PubMed 19592277]).[supplied by OMIM, Feb 2011]
UniProt Code:Q9Y5W3
NCBI GenInfo Identifier:20141620
NCBI Gene ID:10365
NCBI Accession:Q9Y5W3.2
UniProt Secondary Accession:Q9Y5W3,Q6IPC4, Q9UJS5, Q9UKR6,
UniProt Related Accession:Q9Y5W3
Molecular Weight:37,420 Da
NCBI Full Name:Krueppel-like factor 2
NCBI Synonym Full Names:Kruppel like factor 2
NCBI Official Symbol:KLF2  
NCBI Official Synonym Symbols:LKLF  
NCBI Protein Information:Krueppel-like factor 2
UniProt Protein Name:Krueppel-like factor 2
UniProt Synonym Protein Names:Lung krueppel-like factor
Protein Family:Krueppel-like factor
UniProt Gene Name:KLF2  
UniProt Entry Name:KLF2_HUMAN
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Additional Information

Product type:
Antibody
Application:
WB
Application:
IF
Reactivity:
Human
Reactivity:
Mouse
Reactivity:
Rat
Host Species:
Rabbit
Isotype:
IgG
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