KO Validated Antibody
KO Validated Antibodies
What are KO Validated Antibodies?
KO validation is a means by which antibody specificity is confirmed by testing antibodies in a knockout (KO) cell line.
Using KO cell lines (cells with or no expression/reduced expression of precise target proteins) is the new gold standard to validate the specificity of antibodies.
If a signal is detected from a KO cell line, then the antibody is not specific enough. This is due to the antibody binding to a similar protein within the sample. Antibodies with high specificity on the other hand will not produce any signal.
KO validated antibodies must be shown to be specific, selective, and reproducible.
Figure 1: Immunohistochemistry of paraffin-embedded human colon using KO validated FASN antibody (CAB0462) at dilution of 1:100 (40x lens).
Top Antibody Genie KO Validated Antibodies
Why is it important to validate antibodies?
Antibodies are one of the most commonly used research tools. These proteins are used routinely in experiments such as Western blot, ELISA, immunostaining (immunohistochemistry (IHC) and immunocytochemistry (ICC)), immunofluorescence, and flow cytometry among others.
For the findings of these experimental techniques to be reliable they must have an internally high standard of accuracy and precision. This is why there is a need for highly specific antibodies that can ensure reproducibility of results.
Figure 2: Immunofluorescence analysis of HeLa cells using KO validated KRT8 antibody (CAB1024) at dilution of 1:100. Blue: DAPI for nuclear staining.
How do we validate our Knockout Antibodies?
We have created hundreds of CRISPR Knockout cell lines by introducing frame-shift mutations into the genomic DNA of target cells using sgRNA/CAS9.
The CRISPR knockout cells were lysed and Western Blotting was performed using Wild-Type (WT) cells as a control.
The Western Blots were then probed using our premium antibodies. Visualization of decreased protein expression was indicative of highly specific antibodies (Knockout Validated Antibodies).
Figure 3: Schematic representation of how KO validated antibodies are created using CRISPR technology.
Top Validated Antibody targets
| SKU Code | Product |
BCL-10 Knockout Validated Polyclonal Antibody |
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CAPG Knockout Validated Polyclonal Antibody |
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Transgelin-2 Knockout Validated Polyclonal Antibody |
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KRT8 Knockout Validated Polyclonal Antibody |
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GIT1 Knockout Validated Polyclonal Antibody |
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EEF1B2 Knockout Validated Polyclonal Antibody |
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AK2 Knockout Validated Polyclonal Antibody |
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Caspase-3 Knockout Validated Polyclonal Antibody |
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CDK8 Knockout Validated Polyclonal Antibody |
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DNA polymerase beta Knockout Validated Polyclonal Antibody |
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EGFR Knockout Validated Polyclonal Antibody |
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NF2 Knockout Validated Polyclonal Antibody |