[KO Validated] ARHGEF1 Polyclonal Antibody (CAB4274)

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CAB4274
  • Western blot - [KO Validated] ARHGEF1 Polyclonal Antibody (CAB4274)
  • Western blot - [KO Validated] ARHGEF1 Polyclonal Antibody (CAB4274)
  • Immunohistochemistry - [KO Validated] ARHGEF1 Polyclonal Antibody (CAB4274)
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Description

Antibody DatasheetAntibody MSDS

[KO Validated] ARHGEF1 Polyclonal Antibody (CAB4274)

Overview

Title:[KO Validated] ARHGEF1 Polyclonal Antibody (CAB4274)
Size:100µL
Code:CAB4274
Host Species:Rabbit
Purification:Affinity purification
Isotype:IgG

Background

Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined.

Immunogen Information

Immunogen:Recombinant fusion protein containing a sequence corresponding to amino acids 783-912 of human ARHGEF1 (NP_004697.2).
GeneID:9138
SwissProt:Q92888
Synonyms:ARHGEF1; GEF1; LBCL2; LSC; P115-RHOGEF; SUB1.5; rho guanine nucleotide exchange factor 1
Calculated MW:98kDa/102kDa/104kDa/105kDa
Observed MW:102kDa

Applications

Reactivity:Human
Tested Applications:WB; IHC
Recommended Dilution:WB 1:500 - 1:2000; IHC 1:50 - 1:100
Storage Buffer:Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:Western blotting    Immunohistochemistry    
Positive Samples:HeLa, Jurkat, LO2
Cellular Location:Cytoplasm, Membrane

Antibody Images

Western blot - [KO Validated] ARHGEF1 Polyclonal Antibody (CAB4274)

Western blot analysis of extracts of various cell lines, using ARHGEF1 antibody (CAB4274) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 90s.

Western blot - [KO Validated] ARHGEF1 Polyclonal Antibody (CAB4274)

Western blot analysis of extracts from normal (control) and ARHGEF1 knockout (KO) 293T cells, using ARHGEF1 antibody (CAB4274) at 1:500 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

Immunohistochemistry - [KO Validated] ARHGEF1 Polyclonal Antibody (CAB4274)

Immunohistochemistry of paraffin-embedded human tonsil using ARHGEF1 antibody (CAB4274) at dilution of 1:100 (40x lens).

[KO Validated] ARHGEF1 Polyclonal Antibody (CAB4274) Protein Information

UniProt Protein Function:Seems to play a role in the regulation of RhoA GTPase by guanine nucleotide-binding alpha-12 (GNA12) and alpha-13 (GNA13) subunits. Acts as GTPase-activating protein (GAP) for GNA12 and GNA13, and as guanine nucleotide exchange factor (GEF) for RhoA GTPase. Activated G alpha 13/GNA13 stimulates the RhoGEF activity through interaction with the RGS-like domain. This GEF activity is inhibited by binding to activated GNA12. Mediates angiotensin-2-induced RhoA activation.
NCBI Summary:Rho GTPases play a fundamental role in numerous cellular processes that are initiated by extracellular stimuli that work through G protein coupled receptors. The encoded protein may form complex with G proteins and stimulate Rho-dependent signals. Multiple alternatively spliced transcript variants have been found for this gene, but the full-length nature of some variants has not been defined. [provided by RefSeq, Jul 2008]
UniProt Code:Q92888
NCBI GenInfo Identifier:34395524
NCBI Gene ID:9138
NCBI Accession:Q92888.2
UniProt Secondary Accession:Q92888,O00513, Q6NX52, Q8N4J4, Q96BF4, Q96F17, Q9BSB1
UniProt Related Accession:Q92888
Molecular Weight:105,854 Da
NCBI Full Name:Rho guanine nucleotide exchange factor 1
NCBI Synonym Full Names:Rho guanine nucleotide exchange factor 1
NCBI Official Symbol:ARHGEF1  
NCBI Official Synonym Symbols:LSC; GEF1; LBCL2; SUB1.5; P115-RHOGEF  
NCBI Protein Information:rho guanine nucleotide exchange factor 1
UniProt Protein Name:Rho guanine nucleotide exchange factor 1
UniProt Synonym Protein Names:115 kDa guanine nucleotide exchange factor; p115-RhoGEF; p115RhoGEF; Sub1.5
Protein Family:Rho guanine nucleotide exchange factor
UniProt Gene Name:ARHGEF1  
UniProt Entry Name:ARHG1_HUMAN
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Additional Information

Product type:
Antibody
Application:
WB
Application:
IHC
Reactivity:
Human
Host Species:
Rabbit
Isotype:
IgG
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