SPTBN1 Polyclonal Antibody (CAB5253)

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CAB5253

Description
Additional Information

Description

SPTBN1 Polyclonal Antibody (CAB5253)

Overview

Title:	SPTBN1 Polyclonal Antibody (CAB5253)
Size:	100µL
Code:	CAB5253
Host Species:	Rabbit
Purification:	Affinity purification
Isotype:	IgG

Background

Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. It is composed of two antiparallel dimers of alpha- and beta- subunits. This gene is one member of a family of beta-spectrin genes. The encoded protein contains an N-terminal actin-binding domain, and 17 spectrin repeats which are involved in dimer formation. Multiple transcript variants encoding different isoforms have been found for this gene.

Immunogen Information

Immunogen:	Recombinant fusion protein containing a sequence corresponding to amino acids 2100-2200 of human SPTBN1 (NP_003119.2).
GeneID:	6711
SwissProt:	Q01082
Synonyms:	SPTBN1; ELF; HEL102; SPTB2; betaSpII; spectrin beta, non-erythrocytic 1
Calculated MW:	251kDa/253kDa/274kDa
Observed MW:	280kDa

Applications

Reactivity:	Human, Mouse
Tested Applications:	WB
Recommended Dilution:	WB 1:1000 - 1:3000
Storage Buffer:	Store at -20°C. Avoid freeze / thaw cycles.Buffer: PBS with 0.02% sodium azide, 50% glycerol, pH7.3.
Application Key:	Western blotting
Positive Samples:	U-87MG, HeLa, Jurkat, LO2, 293T, Mouse brain
Cellular Location:	Cell membrane, Cytoplasm, Cytoplasmic side, M line, Peripheral membrane protein, cytoskeleton, myofibril, sarcomere

Antibody Images

Western blot analysis of extracts of various cell lines, using SPTBN1 antibody (CAB5253) at 1:3000 dilution.Secondary antibody: HRP Goat Anti-Rabbit IgG (H+L) (CABS014) at 1:10000 dilution.Lysates/proteins: 25ug per lane.Blocking buffer: 3% nonfat dry milk in TBST.Detection: ECL Basic Kit (AbGn00020).Exposure time: 10s.

SPTBN1 Polyclonal Antibody (CAB5253) Protein Information

UniProt Protein Function:	Fodrin, which seems to be involved in secretion, interacts with calmodulin in a calcium-dependent manner and is thus candidate for the calcium-dependent movement of the cytoskeleton at the membrane.
NCBI Summary:	Spectrin is an actin crosslinking and molecular scaffold protein that links the plasma membrane to the actin cytoskeleton, and functions in the determination of cell shape, arrangement of transmembrane proteins, and organization of organelles. It is composed of two antiparallel dimers of alpha- and beta- subunits. This gene is one member of a family of beta-spectrin genes. The encoded protein contains an N-terminal actin-binding domain, and 17 spectrin repeats which are involved in dimer formation. Multiple transcript variants encoding different isoforms have been found for this gene. [provided by RefSeq, Jul 2008]
UniProt Code:	Q01082
NCBI GenInfo Identifier:	116242799
NCBI Gene ID:	6711
NCBI Accession:	Q01082.2
UniProt Secondary Accession:	Q01082,O60837, Q16057, Q53R99, Q59ER3, Q8IX99, B2RP63
UniProt Related Accession:	Q01082
Molecular Weight:	251,397 Da
NCBI Full Name:	Spectrin beta chain, non-erythrocytic 1
NCBI Synonym Full Names:	spectrin beta, non-erythrocytic 1
NCBI Official Symbol:	SPTBN1
NCBI Official Synonym Symbols:	ELF; SPTB2; HEL102; betaSpII
NCBI Protein Information:	spectrin beta chain, non-erythrocytic 1
UniProt Protein Name:	Spectrin beta chain, non-erythrocytic 1
UniProt Synonym Protein Names:	Beta-II spectrin; Fodrin beta chain; Spectrin, non-erythroid beta chain 1
Protein Family:	Spectrin
UniProt Gene Name:	SPTBN1
UniProt Entry Name:	SPTB2_HUMAN